NOS2 Antikörper (AA 1101-1150)

Produktnummer ABIN1533331

Der Kaninchen Polyklonal anti-NOS2 Antikörper (ABIN1533331) detektiert spezifisch NOS2 in WB, ELISA, ICC, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für NOS2 Antikörper (AA 1101-1150) (ABIN1533331)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NOS2 Antikörper

Bindungsspezifität: AA 1101-1150

Verwendungszweck: NOS2 Antibody

Spezifität: NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human iNOS. AA range:1101-1150

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Andere Bezeichnung: iNOS

Hintergrund:

Synonyms: NOS2, NOS2A, Nitric oxide synthase, inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2

Background: function:Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.,induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation.,miscellaneous:Canine and feline parvoviruses bind human and feline transferrin receptors and use these receptors to enter and infect cells.,miscellaneous:Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemia resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.,PTM:N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.,PTM:Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.,PTM:Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).,similarity:Belongs to the peptidase M28 family. M28B subfamily.,similarity:Contains 1 PA (protease associated) domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer, disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC.,

Gene Name: NOS2, INOS

Protein Name: Nitric oxide synthase inducible

Molekulargewicht: 131 kDa

OMIM: 163730

UniProt: P35228

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process