Aconitase 1 Antikörper (pSer138)

Produktnummer ABIN1531675

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Aconitase 1 in IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Aconitase 1 Antikörper (pSer138) (ABIN1531675)

Target: Aconitase 1 (ACO1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aconitase 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Aconitase 1 Antikörper

Bindungsspezifität: AA 106-155, pSer138

Verwendungszweck: IRP-1 (phospho Ser138) Antibody

Spezifität: Phospho-IRP-1 (S138) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S138.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser138. AA range:106-155

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu Aconitase 1

Target: Aconitase 1 (ACO1)

Andere Bezeichnung: IREB1

Hintergrund:

Synonyms: ACO1; IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct

Gene Name: ACO1

Protein Name: Cytoplasmic aconitate hydratase

Molekulargewicht: 98 kDa

Gen-ID: 48

OMIM: 100880

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis