DDIT3 Antikörper (pSer30)

Produktnummer ABIN482168

Der Kaninchen Polyklonal anti-DDIT3 Antikörper wird verwendet zum Nachweis von DDIT3 in Proben von Human und Maus. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für DDIT3 Antikörper (pSer30) (ABIN482168)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDIT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-DDIT3 Antikörper

Bindungsspezifität: AA 15-64, pSer30

Verwendungszweck: CHOP (phospho Ser30) Antibody

Spezifität: Phospho-CHOP (S30) Polyclonal Antibody detects endogenous levels of CHOP protein only when phosphorylated at S30.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CHOP around the phosphorylation site of Ser30. AA range:15-64

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Andere Bezeichnung: CHOP

Hintergrund:

Synonyms: DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, Growth arrest and DNA damage-inducible protein GADD153

Background: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010],

Gene Name: DDIT3

Protein Name: DNA damage-inducible transcript 3 protein

Molekulargewicht: 19 kDa

Gen-ID: 1649

OMIM: 126337

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis