CKS1 Antikörper (C-Term)

Produktnummer ABIN1493317

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CKS1 in WB, ELISA, IF, IHC, IHC (p) und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CKS1 Antikörper (C-Term) (ABIN1493317)

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CKS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-CKS1 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Reacts with the human Cks1 protein. On Western blots, it identifies a single band at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity has been confirmed with HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.

Kreuzreaktivität (Details): Cross-reactivity with mouse is expected based upon amino acid sequence homology.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human and mouse Cks1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF (10 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: 10 μg/mL. Immunoprecipitation: 7 μg/IP reaction. Immunohistochemistry: 2.5 μg/mL. Note: For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, Heat Induced Epitope Retrieval (HIER) with citrate buffer,  pH 6.0, is recommended.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CKS1

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Andere Bezeichnung: CKS1B / CKS1

Hintergrund: Name/Gene ID: CKS1B
Subfamily: CKS

Synonyms: CKS1B, CDC28 protein kinase 1, CKS-1, CDC28 protein kinase 1B, Ckshs1, PNAS-18, PNAS-143, PNAS-16, CDC2-associated protein CKS1, CKS1

Gen-ID: 1163

UniProt: P61024

Pathways: Mitotic G1-G1/S Phases, Inositol Metabolic Process