FBXW7 Antikörper (AA 501-600) (Cy7)

Produktnummer ABIN1416704

Der Kaninchen Polyklonal Anti-FBXW7-Antikörper wurde für WB, IF (p) und IF (cc) validiert. Er ist geeignet, FBXW7 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für FBXW7 Antikörper (AA 501-600) (Cy7) (ABIN1416704)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FBXW7 Antikörper ist konjugiert mit Cy7

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Produktdetails anti-FBXW7 Antikörper (Cy7)

Bindungsspezifität: AA 501-600

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Fbxw7/hCDC4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Andere Bezeichnung: Fbxw7

Hintergrund:

Synonyms: AGO, CDC4, FBW6, FBW7, hAgo, FBX30, FBXW6, SEL10, hCdc4, FBXO30, SEL-10, F-box/WD repeat-containing protein 7, Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, FBXW7

Background: Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463).

Gen-ID: 55294

UniProt: Q969H0

Pathways: Notch Signalweg, EGFR Signaling Pathway