UBE2L3 Antikörper (AA 81-154) (HRP)

Produktnummer ABIN1416442

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch UBE2L3 in WB, ELISA, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für UBE2L3 Antikörper (AA 81-154) (HRP) (ABIN1416442)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2L3 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-UBE2L3 Antikörper (HRP)

Bindungsspezifität: AA 81-154

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Cow,Sheep,Horse,Rabbit,Zebrafish

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ube2L3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Andere Bezeichnung: Ube2L3

Hintergrund:

Synonyms: E2 F1, E2-F1, L UBC, L-UBC, UB2L3_HUMAN, UBCE7, UbcH7, UbcM4, Ube2l3, Ubiquitin carrier protein L3, Ubiquitin conjugating enzyme E2 L3, Ubiquitin protein ligase L3, Ubiquitin-conjugating enzyme E2 L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3.

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2E1 and UBE2L3, also designated UBCH6 and UBCH7 respectively in human, are E2 conjugating enzymes that interact with various proteins. Specifically, UBE2E1 interacts with the tumor suppressor protein TSSC5. UBE2L3 has been shown to mediate c-fos degradation, NF-kB maturation, human papilloma virus-mediated p53 and Myc protein degradation.

Gen-ID: 7332