ARHGEF25/GEFT Antikörper (AA 251-340) (HRP)

Produktnummer ABIN1411556

Der Kaninchen Polyklonal Anti-ARHGEF25/GEFT-Antikörper wurde für WB, ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, ARHGEF25/GEFT in Proben von Ratte zu detektieren.

Kurzübersicht für ARHGEF25/GEFT Antikörper (AA 251-340) (HRP) (ABIN1411556)

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARHGEF25/GEFT Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ARHGEF25/GEFT Antikörper (HRP)

Bindungsspezifität: AA 251-340

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Cow,Sheep,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GEFT

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ARHGEF25/GEFT

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Andere Bezeichnung: GEFT

Hintergrund:

Synonyms: ARHGEF25, ARHGP_HUMAN, GEFT, p63RhoGEF, Guanine nucleotide exchange factor GEFT, p63RhoGEF , RAC/CDC42 exchange factor, Rac/Cdc42/Rho exchange factor GEFT, Rho guanine nucleotide exchange factor 25, RhoA/RAC/CDC42 exchange factor, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT.

Background: GEFT is a 580 amino acid cytoplasmic protein that is highly expressed in excitable tissues such as brain, heart and muscle, and weakly expressed in small intestine, colon, liver, placenta and lung. GEFT may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. GEFT works as a guanine nucleotide exchange factor for the Rho family of small GTPases and links specifically to G alpha q/11-coupled receptors in Rho A activation. GEFT is an important regulator of processes involved in axon and dendrite formation. Involved in skeletal myogenesis, GEFT seems to be an exchange factor primarily for Rac 1 in neurons. Existing as two alternatively spliced variants, GEFT contains a DH (DBL-homology) domain and a PH domain.