BTD Antikörper (AA 401-500) (HRP)

Produktnummer ABIN1411370

Der Kaninchen Polyklonal Anti-BTD-Antikörper wurde für ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, BTD in Proben von Ratte zu detektieren.

Kurzübersicht für BTD Antikörper (AA 401-500) (HRP) (ABIN1411370)

Target: BTD (Biotinidase (BTD))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BTD Antikörper ist konjugiert mit HRP

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-BTD Antikörper (HRP)

Bindungsspezifität: AA 401-500

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Biotinidase

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu BTD

Target: BTD (Biotinidase (BTD))

Andere Bezeichnung: BTD/Biotinidase

Hintergrund:

Synonyms: Biotinase, Biotinidase, Btd, BTD_HUMAN, EC 3.5.1.12.

Background: Biotin, also known as vitamin B7, is an essential water-soluble vitamin that is a cofactor in glucogenesis and in the metabolism of fatty acids and leucine. Biotinidase is a 523 amino acid enzyme that catalyzes the hydrolysis of biocytin to biotin and lysine. Secreted into extracellular space, biotinidase is expressed in liver, heart, placenta, brain, skeletal muscle, pancreas and kidney. Biotinidase contains one carbon-nitrogen hydrolase domain, which is involved in the reduction of organic nitrogen compounds and ammonia production. Defects in the gene encoding biotinidase are the cause of biotinidase deficiency, which is characterized by skin rash, ataxia, seizures, hearing loss, hypotonia and optic atrophy. These symptoms are due to the individual?s inability to reutilize biotin and can, therefore, typically be treated with the addition of free biotin.