Nfasc186 (AA 501-600) Antikörper (HRP)

Produktnummer ABIN1407842

Der Kaninchen Polyklonal anti-Nfasc186 Antikörper wird verwendet zum Nachweis von Nfasc186 in Proben von Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für Nfasc186 (AA 501-600) Antikörper (HRP) (ABIN1407842)

Target: Nfasc186

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails

Bindungsspezifität: AA 501-600

Kreuzreaktivität: Maus, Ratte

Homologie: Human,Dog,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nfasc186

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Nfasc186

Hintergrund:

Synonyms: Neurofascin, NF antibody NF186, NFASC, NRCAML, NFASC_HUMAN.

Background: Nfasc186 (Neurofascin 186) is one of two alternatively spliced products of the Neurofascin (Nfasc) gene in rat, the other being Nfasc155. The establishment of paranodal axoglial junctions in myelinated nerves where voltage-gated sodium channels are concentrated at the nodes of Ranvier is determined by myelin-forming glia. The two isoforms of Neurofascin, Nfasc186 in neurons and Nfasc155 in glia, are required for the assembly of these specialized domains. These two major Neurofascins play essential roles in assembling the nodal and paranodal domains of myelinated axons and are essential for the transition to saltatory conduction in developing vertebrate nerves. Nfasc186 (Isoform 1) is expressed at the Nodes of Ranvier while the Nfasc155 (isoforms 2 and 3) are expressed in unmyelinated axons.