p53 FL-393 (AA 1-393) Antikörper (Biotin)

Produktnummer ABIN1400179

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch in WB, ELISA, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für p53 FL-393 (AA 1-393) Antikörper (Biotin) (ABIN1400179)

Target: p53 FL-393

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: AA 1-393

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Sheep,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: Full length human p53 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Antigendetails

Target: p53 FL-393

Hintergrund:

Synonyms: LFS1, p53, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, Phosphoprotein p53, TP53, Transformation related protein 53, TRP53, Tumor protein p53, Tumour Protein p53.

Background: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Gen-ID: 7157

UniProt: P04637