RIT1 Antikörper (AA 141-219) (FITC)

Produktnummer ABIN1398824

Der Kaninchen Polyklonal Anti-RIT1-Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, RIT1 in Proben von Human zu detektieren.

Kurzübersicht für RIT1 Antikörper (AA 141-219) (FITC) (ABIN1398824)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RIT1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-RIT1 Antikörper (FITC)

Bindungsspezifität: AA 141-219

Homologie: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RIT1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Andere Bezeichnung: RIT1

Hintergrund:

Synonyms: GTP binding protein Roc1, GTP-binding protein Rit1, Ras like protein expressed in many tissues, Ras like without CAAX 1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1, RIBB, Ric like expressed in many tissues, RIT, RIT1, RIT1_HUMAN, ROC1.

Background: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.Neuronal activity dramatically increases the concentration of cytosolic Ca2+, which then serves as a second messenger to direct diverse cellular responses. Calmodulin is a primary mediator of Ca2+ signals in the nervous system. Ric, a protein related to the Ras subfamily of small GTPases, has the ability to bind calmodulin. In addition, two Ras-like human proteins, Rin and Rit (Ric-related gene expressed in many tissues), which are 71 % and 66 % identical to RIC respectively, share related G2 domains with Ric. While most members of the Ras subfamily are plasma membrane-associated and generally require a C-terminal isoprenyl group to bind to the plasma membrane, Rit and Rin lack the recognition signal for C-terminal prenylation. Transiently expressed Rit and Rin are plasma membrane-localized because both proteins contain a C-terminal cluster of basic amino acids, which provides a mechanism for membrane association. Rin binds calmodulin through a C-terminal binding motif. Rit and Ric are widely expressed, whereas expression of Rin is restricted to the neuron system. In conclusion, Rit and Rin define a novel subfamily of Ras-related proteins

Gen-ID: 6016

Pathways: Neurotrophin Signalübertragung