EN2 Antikörper (AA 281-333) (AbBy Fluor® 555)

Produktnummer ABIN1391945

Der Kaninchen Polyklonal Anti-EN2-Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, EN2 in Proben von Human und Maus zu detektieren.

Kurzübersicht für EN2 Antikörper (AA 281-333) (AbBy Fluor® 555) (ABIN1391945)

Target: EN2 (Engrailed Homeobox 2 (EN2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EN2 Antikörper ist konjugiert mit AbBy Fluor® 555

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-EN2 Antikörper (AbBy Fluor® 555)

Bindungsspezifität: AA 281-333

Kreuzreaktivität: Human, Maus

Homologie: Rat,Dog,Sheep,Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EN2/Engrailed 2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu EN2

Target: EN2 (Engrailed Homeobox 2 (EN2))

Andere Bezeichnung: EN2/Engrailed 2

Hintergrund:

Synonyms: Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

Background: The engrailed-2 gene, EN2, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homologs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expression of a-synuclein, a gene that is genetically linked to Parkinson?s disease. During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region. EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia.

Gen-ID: 2020

UniProt: P19622