UBE2E2 Antikörper (AA 1-100)

Produktnummer ABIN1388005

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch UBE2E2 in WB, ELISA, IF (cc), IF (p), IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für UBE2E2 Antikörper (AA 1-100) (ABIN1388005)

Target: UBE2E2 (Ubiquitin-Conjugating Enzyme E2E 2 (UBE2E2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2E2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-UBE2E2 Antikörper

Bindungsspezifität: AA 1-100

Kreuzreaktivität: Human, Ratte

Homologie: Mouse,Cow,Sheep

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2E2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu UBE2E2

Target: UBE2E2 (Ubiquitin-Conjugating Enzyme E2E 2 (UBE2E2))

Andere Bezeichnung: UBE2E2

Hintergrund:

Synonyms: UB2E2_HUMAN, UBC4/5 homolog yeast, UBCH 8, UbcH8, Ube2e2, Ubiquitin carrier protein E2, Ubiquitin conjugating enzyme E2 E2, Ubiquitin conjugating enzyme E2E 2 homologous to yeast UBC4/5, Ubiquitin conjugating enzyme E2E 2 UBC4/5 homolog yeast, Ubiquitin conjugating enzyme E2E 2, Ubiquitin protein ligase E2, Ubiquitin-conjugating enzyme E2 E2, Ubiquitin-protein ligase E2.

Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation.

Gen-ID: 7325