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CD8 alpha Antikörper (AA 51-150)

Name: CD8 alpha Antikörper (AA 51-150)
SKU: ABIN1387948
Price: 357.7 EUR
Availability: InStock

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD8 alpha in FACS, WB, ELISA, IHC (fro), IHC (p), IF (p) und IF (cc). Er zeigt eine Reaktivität gegenüber Human, Ratte und Huhn und wurde in 2+ Publikationen erwähnt.

Paraformaldehyde-fixed, paraffin embedded rat spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (CD8 alpha Antikörper (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (ABIN1387948) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD8 alpha Antikörper (AA 51-150))

IF (p)

3 Abbildungen

(2 Referenzen)

Produktnummer ABIN1387948

Kurzübersicht für CD8 alpha Antikörper (AA 51-150) (ABIN1387948)

Target

Alle CD8 alpha (CD8A) Antikörper anzeigen

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität

Human, Ratte, Huhn

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Discover our top product CD8A Primärantikörper

Bindungsspezifität
15

13

12

11

9

9

9

9

5

5

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150

Kreuzreaktivität

Huhn, Human, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from rat CD8 alpha

Isotyp

IgG
anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A Reaktivität: Human FACS, WB, ELISA Wirt: Maus Monoclonal 8H8A8 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reaktivität: Maus FACS, IP, IHC (fro), IHC (p), ICC, Func Wirt: Ratte Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody (FITC)
CD8A Reaktivität: Maus FACS Wirt: Ratte Monoclonal 53-6-7 FITC

anti-CD8a Molecule (CD8A) antibody
CD8A Reaktivität: Maus FACS, IP, IHC (fro), IHC (p), ICC Wirt: Ratte Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody (PE)
CD8A Reaktivität: Maus FACS Wirt: Ratte Monoclonal 53-6-7 PE

anti-CD8a Molecule (CD8A) antibody
CD8A Reaktivität: Human IHC, WB, IP, ELISA Wirt: Kaninchen Monoclonal 2F2 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) (AA 22-182) antibody
CD8A Reaktivität: Human FACS, ELISA Wirt: Maus Monoclonal 6A9A5 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reaktivität: Human IHC, StM, ISt Wirt: Maus Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A Reaktivität: Maus, Ratte FACS, IHC, WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (AA 150-180) antibody
CD8A Reaktivität: Human FACS, IHC, WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).
Target

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung

CD8 alpha

Hintergrund

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gen-ID

24930

UniProt

P07725

Pathways

T-Zell Rezeptor Signalweg