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CD19 Antikörper (AA 151-250)

Name: CD19 Antikörper (AA 151-250)
SKU: ABIN1387313
Price: 357.7 EUR
Availability: InStock

Der Kaninchen Polyklonal Anti-CD19-Antikörper wurde für FACS, WB, ELISA und IHC (p) validiert. Er ist geeignet, CD19 in Proben von Human, Maus und Ratte zu detektieren.

Mouse splenocytes probed with Rabbit Anti-CD19 Polyclonal Antibody, PE-Cy5 Conjugated . (CD19 Antikörper (AA 151-250))

FACS

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm. (CD19 Antikörper (AA 151-250))

ELISA

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD19 Polyclonal Antibody, Unconjugated (ABIN1387313) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD19 Antikörper (AA 151-250))

IHC

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C. (CD19 Antikörper (AA 151-250))

IF (p)

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (CD19 Antikörper (AA 151-250))

Paraformaldehyde-fixed, paraffin embedded fetal mouse brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 Antikörper (AA 151-250))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 Antikörper (AA 151-250))

IHC (p)

7 Abbildungen

Produktnummer ABIN1387313

Kurzübersicht für CD19 Antikörper (AA 151-250) (ABIN1387313)

Target

Alle CD19 Antikörper anzeigen

CD19 (CD19 Molecule (CD19))

Reaktivität

Human, Maus, Ratte

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser CD19 Antikörper ist unkonjugiert

Applikation

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Bindungsspezifität
25

21

16

15

14

12

10

7

7

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-250

Kreuzreaktivität

Human, Maus, Ratte

Homologie

Pig,Guinea Pig

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD19

Isotyp

IgG
anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 Reaktivität: Human FACS, WB, IHC, IF, ELISA Wirt: Maus Monoclonal 8G11B7 unconjugated

anti-CD19 Molecule (CD19) (C-Term) antibody
CD19 Reaktivität: Human, Maus, Ratte, Affe, Schaf, Schwein, Rind (Kuh), Meerschweinchen, Kaninchen, Hund, Avian, Cat, Huhn, Esel, Ziege, Hamster, Pferd WB Wirt: Ziege Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 485-556) antibody
CD19 Reaktivität: Human, Maus, Ratte, Schaf FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD19 Molecule (CD19) (pTyr531) antibody
CD19 Reaktivität: Human, Maus FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reaktivität: Human FACS, WB, IF, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 Reaktivität: Human FACS, IHC, ELISA, Coat, StM Wirt: Maus Monoclonal CD19-3116 unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 Reaktivität: Human FACS, WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reaktivität: Human FACS, ICC Wirt: Maus Monoclonal 4G7 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reaktivität: Human FACS, IP Wirt: Maus Monoclonal LT19 unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

CD19 (CD19 Molecule (CD19))

Andere Bezeichnung

CD19

Hintergrund

Synonyms: B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Gen-ID

930

UniProt

P15391

Pathways

Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung