WT1 Antikörper (AA 301-400)

Produktnummer ABIN1386293

Der Kaninchen Polyklonal Anti-WT1-Antikörper wurde für FACS, WB, ELISA, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, WT1 in Proben von Human, Ratte, Maus und Rind (Kuh) zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für WT1 Antikörper (AA 301-400) (ABIN1386293)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-WT1 Antikörper

Bindungsspezifität: AA 301-400

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Homologie: Dog,Cow,Sheep,Pig,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Wilms Tumor Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-WT1 Antikörper (ABIN1386293)

Xiao, Guan, Nie, Wang, Sun, He, Huang, Zhang, Yang, Wang, Zhao: "Rapamycin promotes podocyte autophagy and ameliorates renal injury in diabetic mice." in: Molecular and cellular biochemistry, Vol. 394, Issue 1-2, pp. 145-54, (2014) (PubMed).

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: Wilms Tumor Protein

Hintergrund:

Synonyms: GUD, AWT1, WAGR, WT33, NPHS4, WIT-2, EWS-WT1, Wilms tumor protein, WT1

Background: Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Molekulargewicht: 55kDa

Gen-ID: 7490

UniProt: P19544

Pathways: Tube Formation