p300 Antikörper (AA 1451-1600)

Produktnummer ABIN1386187

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch p300 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für p300 Antikörper (AA 1451-1600) (ABIN1386187)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p300 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-p300 Antikörper

Bindungsspezifität: AA 1451-1600

Kreuzreaktivität: Human, Maus

Homologie: Rat,Dog,Cow,Sheep,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EP300 inhibitory protein 1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Andere Bezeichnung: Ep300

Hintergrund:

Synonyms: CREBBP/EP300 inhibitory protein 1, Cyclic AMP responsive enhancer binding protein, E1A associated protein p300, E1A binding protein p300, E1A-associated protein p300, EC 2.3.1.48, EP300, EP300: E1A binding protein p300, EP300_HUMAN, istone acetyltransferase p300, 300 HAT, RB and P300 binding protein EID 1, Retinoblastoma protein associated protein, RSTS2.

Background: Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 Molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

Pathways: p53 Signalweg, Notch Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size