NSF Antikörper (AA 151-250)

Produktnummer ABIN1385801

Produktdetails anti-NSF Antikörper

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Bindungsspezifität: AA 151-250

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NSF

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Andere Bezeichnung: NSF (NSF Produkte)

Synonyme: SKD2 antikoerper, AI316878 antikoerper, AU020090 antikoerper, AU067812 antikoerper, NSF antikoerper, 18.m06598 antikoerper, nsf antikoerper, si:bz18k17.1 antikoerper, wu:fj33g11 antikoerper, N-ethylmaleimide sensitive factor antikoerper, T1J1.4 antikoerper, T1J1_4 antikoerper, NEM-sensitive fusion protein antikoerper, N-ethylmaleimide sensitive factor, vesicle fusing ATPase antikoerper, N-ethylmaleimide sensitive fusion protein antikoerper, N-ethylmaleimide-sensitive factor antikoerper, N-ethylmaleimide sensitive factor antikoerper, N-ethylmaleimide-sensitive fusion protein, putative antikoerper, N-ethylmaleimide sensitive factor L homeolog antikoerper, vesicle-fusing ATPase antikoerper, N-ethylmaleimide-sensitive factor a antikoerper, AAA-type ATPase family protein antikoerper, NSF antikoerper, Nsf antikoerper, nsf antikoerper, BBOV_II007200 antikoerper, TGME49_318510 antikoerper, nsf.L antikoerper, LOC100180685 antikoerper, LOC9310886 antikoerper, nsfa antikoerper

Hintergrund:

Synonyms: N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor like protein, N ethylmaleimide sensitive fusion protein, N ethylmaleimide sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, NEM sensitive fusion protein, NEM sensitive fusion protein, NEM-sensitive fusion protein, NSF, NSF_HUMAN, SKD 2, SKD2, SKD2, Vesicle fusing ATPase, Vesicle fusing ATPase, Vesicle-fusing ATPase, Vesicular fusion protein NSF, Vesicular fusion protein NSF, Vesicular-fusion protein NSF.

Background: Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the -SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the -SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).