NSMCE2 Antikörper

Produktnummer ABIN1385784

Dieses Anti-NSMCE2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NSMCE2 in WB, IHC (p) und IF (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für NSMCE2 Antikörper (ABIN1385784)

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSMCE2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-NSMCE2 Antikörper

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MMS21/NSE2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu NSMCE2

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Andere Bezeichnung: MMS21

Hintergrund:

Synonyms: C8orf36, E3 SUMO-protein ligase NSE2, hMMS21, hNSE2, methyl methanesulfonate sensitivity gene 21, MMS21, MMS21 homolog, non-SMC element 2, Non-SMC element 2 homolog, Non-structural maintenance of chromosomes element 2 homolog, NSE2_HUMAN, NSMCE2.

Background: Breaks in double stranded DNA often arise during DNA replication or as a result of exposure to DNA-damaging agents. Quick and accurate repair of these breaks is crucial for cell survival and genomic stability. Structural maintenance of chromosomes (SMC) family members form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation during mitosis. SMC5 and SMC6 play a crucial role in DNA repair as they form a complex with six conserved nonSMC subunits, including a ubiquitin E3 ligase NSE1 and a SUMO ligase NSE2. Specifically, this complex is crucial for sister chromatid homologous recombination DNA repair and also for prevention of chromosomal rearrangements. The NSE1 protein contains a RING-like motif that promotes DNA repair functions of the SMC5/SMC6 complex and full deletion of NSE1 is lethal to cells. NSE2 stimulates sumoylation of SMC6 and the DNA repair protein TRAX. Depletion of the NSE2 protein by RNA interference leaves the cell vulnerable to DNA damage-induced apoptosis.

Gen-ID: 286053