CLEC14A Antikörper (AA 260-310)

Produktnummer ABIN1385496

Dieses Anti-CLEC14A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CLEC14A in WB, IF (p) und IHC (p). Geeignet für Human, Maus und Ratte. Unabhängig validiert für die Verwendung in Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections).

Kurzübersicht für CLEC14A Antikörper (AA 260-310) (ABIN1385496)

Target: CLEC14A (C-Type Lectin Domain Family 14, Member A (CLEC14A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLEC14A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CLEC14A Antikörper

Bindungsspezifität: AA 260-310

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC (fp))

Validierung #100004 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100004

Datum: 23.11.2015

Antigen: CLEC14A

Validierte Anwendung: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Positivkontrolle: Tumor cell transplanted mouse brain was dissected

Validierungsbilder

IF validation of CLEC14A antibody ABIN1385496 on mouse brain tumor sections. A. ABIN1385496 diluted 1:100 (blue), DAPI counterstaining (red). B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.

Protokoll

Primärantikörper: ABIN1385496

Sekundärantikörper: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• Dry slides at 50-55°C for 15-30 min (or RT for 30min)
• Wash three times with PBS 5min (shake gently)
• Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
• Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
• Rabbit-anti-CLEC14A antibody (ABIN1385496) diluted 1:50-1:400
• Wash three times with PBS 5min (shake gently)
• Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
• Donkey-anti-Rabbit-Cy3
• DAPI 1:100
• Wash three times with PBS 5min (shake gently)
• Add three drops of water-based Immu-mount
• Cover with 24x60 cover glass
• Imaging
• Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Anmerkungen: ABIN1385496 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu CLEC14A

Target: CLEC14A (C-Type Lectin Domain Family 14, Member A (CLEC14A))

Andere Bezeichnung: EGFR5

Hintergrund:

Synonyms: CEG1, EGFR-5, C14orf27, C-type lectin domain family 14 member A, Epidermal growth factor receptor 5, CLEC14A, EGFR5, UNQ236/PRO269

Background: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation, cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium, it is considered to be a candidate for tumor vascular targeting.

Gen-ID: 161198

UniProt: Q86T13