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CD8 Antikörper (AA 51-150)

Name: CD8 Antikörper (AA 51-150)
SKU: ABIN1385272
Price: 384.62 EUR
Availability: InStock

CD8 Reaktivität: Human FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

U251 cells probed with CD8 Polyclonal Antibody, Unconjugated for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (CD8 Antikörper (AA 51-150))

FACS

Protein: line1,mouse lung lysates; line2,mouse testicle lysates; Primary: Anti-CD8 at 1: 5000; Predicted band size:27 kD Observed band size:48 kD (CD8 Antikörper (AA 51-150))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN1385272) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 (CD8 Antikörper (AA 51-150))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD8 Polyclonal Antibody, Unconjugated (ABIN1385272) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD8 Antikörper (AA 51-150))

IHC

4 Abbildungen

(3 Referenzen)

Produktnummer ABIN1385272

Datenblatt (PDF)

Target Alle CD8 Antikörper anzeigen

CD8
Bindungsspezifität
21

19

17

4

3

2

1

1

1

1

1
AA 51-150
Reaktivität
209

66

25

12

12

12

9

9

8

8

6

6

6

5

5

5

4

4

3

2

1

1
Human
Wirt
243

48

36

4
Kaninchen
Passende Sekundärantikörper
Klonalität
294

30

2
Polyklonal
Konjugat
106

49

38

26

13

7

5

5

5

5

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD8 Antikörper ist unkonjugiert
Applikation
288

68

55

52

50

46

32

21

18

17

15

14

8

5

5

4

3

2

2

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität

Human

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD8

Isotyp

IgG

Top Product

Discover our top product CD8 Primärantikörper
anti-CD8 (CD8) (AA 51-150) antibody
CD8 Reaktivität: Human, Maus, Ratte FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-31 PE

anti-CD8 (CD8) (AA 22-187) antibody
CD8 Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD8 (CD8) antibody
CD8 Reaktivität: Human FACS, IP, CyTOF Wirt: Maus Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (Biotin)
CD8 Reaktivität: Human FACS, IP Wirt: Maus Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody
CD8 Reaktivität: Human IHC, IP, WB, ELISA Wirt: Kaninchen Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody (PerCP)
CD8 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-31 APC

anti-CD8 (CD8) antibody (PE)
CD8 Reaktivität: Cat FACS Wirt: Maus Monoclonal fCD8 PE

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Zou, Guo, Lv, Huang, Li, Wang, Jiang, She: "Clinicopathologic implications of CD8+/Foxp3+ratio and miR-574-3p/PD-L1 axis in spinal chordoma patients." in: Cancer immunology, immunotherapy : CII, Vol. 67, Issue 2, pp. 209-224, (2017) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Mongkhoune, Xie, Wang, Chen, Zhou, Peng, He, Yu, Zhou, Luo, Zhao, Lu: "A constructed HLA-A2-restricted pMAGE-A1(278-286) tetramer detects specific cytotoxic T lymphocytes in tumour tissues in situ." in: The Journal of international medical research, Vol. 41, Issue 6, pp. 1811-24, (2013) (PubMed).
Target

CD8

Andere Bezeichnung

CD8 (CD8 Produkte)

Hintergrund

Synonyms: CD8, MAL, p32, Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gen-ID

925

UniProt

P01732