AAV VP1 Antikörper

Produktnummer ABIN1385060

This anti-AAV VP1 antibody (ABIN1385060) is a Rabbit Polyclonal antibody detecting AAV VP1 in IF (cc), IF (p), ELISA, IHC (fro), IHC (p), ICC. Suitable for Adeno-Associated Virus (AAV).

Kurzübersicht für AAV VP1 Antikörper (ABIN1385060)

Target: AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))

Reaktivität: Adeno-Associated Virus (AAV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AAV VP1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-AAV VP1 Antikörper

Spezifität: This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13

Kreuzreaktivität: Virus

Kreuzreaktivität (Details): AAV5

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu AAV VP1

Target: AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))

Andere Bezeichnung: Adeno-Associated Virus Capsid Protein VP1

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.

Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus