CD28 Antikörper

Produktnummer ABIN135532

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD28 in FACS. Er zeigt eine Reaktivität gegenüber Huhn und wurde in 1 Publikation erwähnt.

Kurzübersicht für CD28 Antikörper (ABIN135532)

Target: CD28

Reaktivität: Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: AV7

Produktdetails anti-CD28 Antikörper

Spezifität: Chicken/Turkey CD28, Mr 40-44 kDa

Produktmerkmale: Mouse Anti-Chicken CD28-UNLB

Aufreinigung: Purified

Immunogen: Chicken peripheral blood lymphocytes

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Costim - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Kommentare:

In vitro T-cell costimulation

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Konservierungsmittel: Without preservative

Handhabung: Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen für anti-CD28 Antikörper (ABIN135532)

Cha, Khatri, Mutnal, Sharma: "Pathogenic and immunogenic responses in turkeys following in ovo exposure to avian metapneumovirus subtype C." in: Veterinary immunology and immunopathology, (2010) (PubMed).

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V1- and V2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response