L-Selectin Antikörper (Biotin)

Produktnummer ABIN135251

Der Ratte Monoklonal Anti-L-Selectin-Antikörper wurde für FACS validiert. Er ist geeignet, L-Selectin in Proben von Maus zu detektieren.

Kurzübersicht für L-Selectin Antikörper (Biotin) (ABIN135251)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser L-Selectin Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: MEL-14

Produktdetails anti-L-Selectin Antikörper (Biotin)

Spezifität: Mouse CD62L, Mr 74 kDa on lymphocytes and Mr 95 kDa on neutrophils

Produktmerkmale: Rat Anti-Mouse CD62L-BIOT

Aufreinigung: Ion exchange column

Immunogen: C3H/eb cloned mouse B cell lymphoma 38C-13

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PACBLU conjugate 0.3 g/106 cells PE, APC, SPRD, PE/CY7, APC/CY7, AF488, AF647, and AF700 0.1 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Kommentare:

In vivo and in vitro blocking of adhesion, Cytotoxicity studies

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Details zu L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Andere Bezeichnung: CD62L

Substanzklasse: Chemical

Hintergrund: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.