HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) Antikörper (Biotin)

Details zu Produkt Nr. ABIN1302714
Antigen
Reaktivität
Human
130
8
7
6
6
2
2
1
1
Wirt
Maus
66
65
Klonalität (Klon)
Monoklonal ()
Konjugat
Biotin
11
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
70
41
32
24
24
20
13
2
2
1
Optionen
Immunogen Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Klon MEM-E-07
Isotyp IgG1
Spezifität The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Kreuzreaktivität (Details) Human
Antigen
Andere Bezeichnung HLA-E (HLAE Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Applikations-hinweise Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 2 microgram/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Kommentare

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) antibody (Biotin) (ABIN1302714) Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (cl...
Produkt verwendet in: Nachmani, Zimmermann, Oiknine Djian, Weisblum, Livneh, Khanh Le, Galun, Horejsi, Isakov, Shomron, Wolf, Hengel, Mandelboim: "MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, 2014 (PubMed).

Lo Monaco, Tremante, Cerboni, Melucci, Sibilio, Zingoni, Nicotra, Natali, Giacomini: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, 2011 (PubMed).