SCIMP Antikörper

Produktnummer ABIN1302508

Dieser Maus Monoklonal Antikörper detektiert spezifisch SCIMP in IP, WB, ICC und ICFC. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für SCIMP Antikörper (ABIN1302508)

Target: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SCIMP Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Klon: NVL-07

Produktdetails anti-SCIMP Antikörper

Verwendungszweck: Anti-SCIMP Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant intracellular part of human SCIMP

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-SCIMP Antikörper (ABIN1302508)

Draber, Vonkova, Stepanek, Hrdinka, Kucova, Skopcova, Otahal, Angelisova, Horejsi, Yeung, Weiss, Brdicka: "SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling." in: Molecular and cellular biology, Vol. 31, Issue 22, pp. 4550-62, (2011) (PubMed).

Details zu SCIMP

Target: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Andere Bezeichnung: SCIMP

Hintergrund: SLP adaptor and CSK interacting membrane protein,SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.,NVL

Gen-ID: 388325

UniProt: Q6UWF3