The polyclonal antibody reacts with intracellular antigens MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation.
Aufreinigung
Purified by ligand affinity chromatography.
Reinheit
> 95 % (by SDS-PAGE)
Immunogen
Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).
Western blotting: Recommended dilution 0.5 μg/mL, positive material: RAJI human Burkitt lymphoma cell line, both reducing and non-reducing conditions, reducing conditions are recommended.
MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade –, an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.,MAPKK 1/2, MKK1/2