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MEK1/2 Antikörper

Der Kaninchen Polyklonal Anti-MEK1/2-Antikörper wurde für WB validiert. Er ist geeignet, MEK1/2 in Proben von Human zu detektieren.
Produktnummer ABIN1302344

Kurzübersicht für MEK1/2 Antikörper (ABIN1302344)

Target

MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reaktivität

  • 75
  • 71
  • 71
  • 10
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 75
  • 1
Kaninchen

Klonalität

  • 66
  • 10
Polyklonal

Konjugat

  • 21
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser MEK1/2 Antikörper ist unkonjugiert

Applikation

  • 63
  • 27
  • 27
  • 20
  • 11
  • 6
  • 6
  • 2
  • 1
  • 1
Western Blotting (WB)
  • Verwendungszweck

    Anti-MEK1/2 Purified

    Spezifität

    The polyclonal antibody reacts with intracellular antigens MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation.

    Aufreinigung

    Purified by ligand affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).

    Isotyp

    IgG
  • Applikationshinweise

    Western blotting: Recommended dilution 0.5 μg/mL, positive material: RAJI human Burkitt lymphoma cell line, both reducing and non-reducing conditions, reducing conditions are recommended.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Target

    MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

    Andere Bezeichnung

    MEK1/2

    Hintergrund

    MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade –, an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.,MAPKK 1/2, MKK1/2
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