Vesicular Stomatitis Virus G Protein (VSV G) (C-Term) Antikörper (Biotin)

Produktnummer ABIN129734

Hersteller Produkt- Nr.: 600-406-386

Produktdetails

Target: Vesicular Stomatitis Virus G Protein (VSV G)

Bindungsspezifität: C-Term

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 600-406-386

Hersteller: Rockland

Verwendungszweck: VSV-G Epitope Tag Antibody Biotin Conjugated

Sequenz: YTDIEMNRLG K

Kreuzreaktivität (Details): This affinity purified antibody is directed against VSV-G protein.

Produktmerkmale: Synonyms: Rabbit Anti-VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti-VSV-G Tag Antibody Biotin Conjugation

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification followed by biotin conjugation, subsequent purification and exhaustive dialysis against the buffer stated above.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot and is suitable by immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user.  For standard indirect immunofluorescence assays, we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein.  Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200.   At higher concentrations, a filamentous background staining may be present.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: Vesicular Stomatitis Virus G Protein (VSV G)

Andere Bezeichnung: VSV-G

Substanzklasse: Viral Protein

Hintergrund: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.   VSV-G, or vesicular stomatitis virus glycoprotein, is found within the pseudo lentiviral cloning vector pH CMV-VSV-G.