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Aubergine Antikörper (Internal Region)

Dieses Anti-Aubergine-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Aubergine in ELISA und WB. Geeignet für Drosophila melanogaster.
Rockland
Produktnummer ABIN129699
Hersteller Produkt- Nr.: 600-401-947

Kurzübersicht für Aubergine Antikörper (Internal Region) (ABIN129699)

Target

Aubergine (aub)

Reaktivität

Drosophila melanogaster

Wirt

Kaninchen

Klonalität

Polyklonal

Applikation

ELISA, Western Blotting (WB)
  • Bindungsspezifität

    Internal Region

    Hersteller Produkt- Nr.

    600-401-947

    Hersteller

    Rockland

    Verwendungszweck

    Aubergine/Sting Antibody

    Kreuzreaktivität (Details)

    Anti-Aubergine affinity-purified antibody is directed against Drosophila Aubergine protein.

    Produktmerkmale

    Synonyms: rabbit anti-Aubergine/Sting Antibody, rabbit anti-Aubergine Antibody, rabbit anti-Sting Antibody, Aubergine isoform A

    Aufreinigung

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Drosophila melanogaster Aubergine/Sting protein.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: Aubergine affinity-purified antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 98 kDa in size corresponding to Aubergine protein is expected in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000 - 1:10,000

    ELISA Dilution: 1:20,000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    Aubergine (aub)

    Andere Bezeichnung

    aub

    Substanzklasse

    Species

    Hintergrund

    Background: Aubergine (Sting) is a Drosophila protein involved in germ-line development and RNA interference.  Drosophila melanogaster has a robust and efficient innate immune system, which reacts to infections ranging from bacteria to fungi and, as discovered recently, viruses as well. The known Drosophila immune responses rely on humoral and cellular activities, similar to those found in the innate immune system of other animals. Recently, RNAi or 'RNA silencing' has arisen as a possible means by which Drosophila can react to specific pathogens, transposons and retroviral elements, in a fashion similar to that of a traditional mammalian adaptive immune system instead of in a more generalized and genome encoded innate immune-based response. RNAi is a highly conserved regulation and defense mechanism, which suppresses gene expression via targeted RNA degradation directed by either exogenous dsRNA (cleaved into siRNAs) or endogenous miRNAs. In plants, RNAi has been found to act as an antiviral immune response system. In Drosophila multiple core RNAi pathway genes have been reported, including piwi, vasa intronic gene (vig), aubergine (aub), armitage (armi), Rm62, r2d2 and Argonaute2 (AGO2).

    Gen-ID

    34524, 17136494

    UniProt

    O76922
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