ASAP1 Antikörper (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1) pTyr782

Produktnummer ABIN129669

Produktdetails anti-ASAP1 Antikörper

Target: ASAP1

Bindungsspezifität: AA 777-787, pTyr782

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASAP1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Kreuzreaktivität: Human, Huhn, Schaf, Hund, Ratte (Rattus), Schimpanse

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 777-787 of mouse ASAP1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, IF microscopy and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated ASAP1 protein by western blotting in the appropriate cell lysate or extract.  Less than 2.5% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pY782 of ASAP1 protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.09 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-ASAP1 Antikörper (ABIN129669)

Bender, Stritt, Nurden, van Eeuwijk, Zieger, Kentouche, Schulze, Morbach, Stegner, Heinze, Heinze, Dütting, Gupta, Witke, Falet, Fischer, Hartwig, Nieswandt: "Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott-Aldrich syndrome-like platelet defect." in: Nature communications, Vol. 5, pp. 4746, (2015) (PubMed).

Details zu ASAP1

Target: ASAP1

Andere Bezeichnung: ASAP1 (ASAP1 Produkte)

Synonyme: AV239055, DEF-1, Ddef1, PAP, mKIAA1249, s19, AMAP1, CENTB4, DDEF1, PAG2, ZG14P, ASAP1, ddef1, Asap1, si:ch211-160i2.3, ArfGAP with SH3 domain, ankyrin repeat and PH domain1, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1, toxic extracellular endopeptidase, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 L homeolog, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1a, Asap1, ASAP1, asaP1, asap1, asap1.L, asap1a

Hintergrund: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes.  Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.
Synonyms: Development and differentiation enhancing factor 1 antibody, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody, ADP ribosylation factor directed GTPase activating protein 1 antibody, AMAP 1 antibody

Gen-ID: 13196, 65301464

UniProt: Q9QWY8