ASAP1 Antikörper (AA 775-800)

Produktnummer ABIN129669

Hersteller Produkt- Nr.: 600-401-910

Produktdetails anti-ASAP1 Antikörper

Target: ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))

Bindungsspezifität: AA 775-800

Reaktivität: Maus, Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-910

Hersteller: Rockland

Verwendungszweck: Asap1 phospho Y782 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against the phosphorylated form of mouse ASAP1 protein at the pY782 residue.

Produktmerkmale: Synonyms: rabbit anti-ASAP1 pY782 Antibody, ASAP-1, ASAP 1, Development and differentiation enhancing factor 1 antibody, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody, ADP ribosylation factor directed GTPase activating protein 1 antibody, AMAP 1 antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 20-40 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, IF microscopy and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated ASAP1 protein by western blotting in the appropriate cell lysate or extract.  Less than 2.5 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pY782 of ASAP1 protein.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:16,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.09 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ASAP1 Antikörper (ABIN129669)

Bender, Stritt, Nurden, van Eeuwijk, Zieger, Kentouche, Schulze, Morbach, Stegner, Heinze, Heinze, Dütting, Gupta, Witke, Falet, Fischer, Hartwig, Nieswandt: "Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott-Aldrich syndrome-like platelet defect." in: Nature communications, Vol. 5, pp. 4746, (2015) (PubMed).

Details zu ASAP1

Target: ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))

Andere Bezeichnung: Asap1 (ASAP1 Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130- kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes.  Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.

Gen-ID: 13196, 65301464

UniProt: Q9QWY8