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NOXO1 Antikörper (AA 238-252)

Der Kaninchen Polyklonal Anti-NOXO1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, NOXO1 in Proben von Human zu detektieren.
Rockland
Produktnummer ABIN129665
Hersteller Produkt- Nr.: 600-401-899

Kurzübersicht für NOXO1 Antikörper (AA 238-252) (ABIN129665)

Target

Alle NOXO1 Antikörper anzeigen
NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reaktivität

  • 22
  • 1
  • 1
  • 1
Human

Wirt

  • 20
  • 1
  • 1
Kaninchen

Klonalität

  • 22
Polyklonal

Konjugat

  • 14
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser NOXO1 Antikörper ist unkonjugiert

Applikation

  • 17
  • 13
  • 3
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 238-252

    Hersteller Produkt- Nr.

    600-401-899

    Hersteller

    Rockland

    Verwendungszweck

    NOXO1 Antibody

    Kreuzreaktivität (Details)

    Anti-NOXO-1 antibody is directed against human NOXO1 protein.

    Produktmerkmale

    Synonyms: rabbit anti-NOXO1 antibody, NOXO-1, NOXO 1, NADPH oxidase organizer 1 antibody, NADPH oxidase regulatory protein antibody, Nox organizer 1 antibody, Nox organizing protein 1 antibody, Regulatory protein P41NOX antibody, SH3 and PX domain-containing protein 5, P41NOX, SH3PXD5

    Aufreinigung

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 5-10 μg/mL

    Application Note: Anti-NOXO1 antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000 - 1:10,000

    ELISA Dilution: 1:20,000 - 1:100,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.17 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

    Andere Bezeichnung

    NOXO1

    Hintergrund

    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells.  NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region.  NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.

    Gen-ID

    124056, 16198473

    UniProt

    Q8NFA2
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