VDAC1 Antikörper (Voltage-Dependent Anion Channel 1) Internal Region

Produktnummer ABIN129654

Produktdetails anti-VDAC1 Antikörper

Target: VDAC1

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VDAC1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).

Produktmerkmale: VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

Sterilität: Sterile filtered

Immunogen: VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 185-197 of Human VDAC1/Porin1.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: VDAC/Porin Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: VDAC1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-VDAC1 Antikörper (ABIN129654)

Thakurela, Garding, Jung, Müller, Goebbels, White, Werner, Tiwari: "The transcriptome of mouse central nervous system myelin." in: Scientific reports, Vol. 6, pp. 25828, (2018) (PubMed).

Petermann, Haase, Knebel-Mörsdorf: "Impact of Rac1 and Cdc42 signaling during early herpes simplex virus type 1 infection of keratinocytes." in: Journal of virology, Vol. 83, Issue 19, pp. 9759-72, (2009) (PubMed).

Bourekas, Slivka, Shah, Sunshine, Suarez: "Intraarterial thrombolytic therapy within 3 hours of the onset of stroke." in: Neurosurgery, Vol. 54, Issue 1, pp. 39-44; discussion 44-6, (2003) (PubMed).

Details zu VDAC1

Target: VDAC1

Andere Bezeichnung: VDAC/Porin (VDAC1 Produkte)

Synonyme: vdac1, VDAC1, 5076, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1, ATVDAC1, T22N4.9, T22N4_9, voltage dependent anion channel 1, PORIN, VDAC-1, AL033343, Vdac5, fa13f11, wu:fa13f11, zgc:85830, voltage-dependent anion channel 1 S homeolog, voltage-dependent anion channel 1, voltage dependent anion channel 1, vdac1.S, vdac1, VDAC1, Vdac1

Hintergrund: VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

Gen-ID: 130683

UniProt: Q9Y277