VDAC1 Antikörper (AA 175-200)

Produktnummer ABIN129654

Hersteller Produkt- Nr.: 600-401-882

Produktdetails anti-VDAC1 Antikörper

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Bindungsspezifität: AA 175-200

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Hersteller Produkt- Nr.: 600-401-882

Hersteller: Rockland

Verwendungszweck: VDAC/Porin Antibody

Kreuzreaktivität (Details): Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein.

Produktmerkmale: Synonyms: rabbit anti-VDAC/Porin antibody, VDAC-1 Loading Control Antibody, Porin Loading Control Antibody, VDAC-1 Antibody, VDAC1 Antibody, POR1 Antibody, Porin Antibody, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: VDAC/Porin Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:30,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store VDAC antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-VDAC1 Antikörper (ABIN129654)

Thakurela, Garding, Jung, Müller, Goebbels, White, Werner, Tiwari: "The transcriptome of mouse central nervous system myelin." in: Scientific reports, Vol. 6, pp. 25828, (2018) (PubMed).

Petermann, Haase, Knebel-Mörsdorf: "Impact of Rac1 and Cdc42 signaling during early herpes simplex virus type 1 infection of keratinocytes." in: Journal of virology, Vol. 83, Issue 19, pp. 9759-72, (2009) (PubMed).

Bourekas, Slivka, Shah, Sunshine, Suarez: "Intraarterial thrombolytic therapy within 3 hours of the onset of stroke." in: Neurosurgery, Vol. 54, Issue 1, pp. 39-44; discussion 44-6, (2003) (PubMed).

Details zu VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Andere Bezeichnung: VDAC1 (VDAC1 Produkte)

Hintergrund: Background: VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below).  The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane.  VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.  VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.  This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

Gen-ID: 7416

NCBI Accession: NP_003365

UniProt: P21796