MAD2L1 Antikörper (AA 1-25)

Produktnummer ABIN129587

Hersteller Produkt- Nr.: 600-401-461

Der Kaninchen Polyklonal Anti-MAD2L1-Antikörper wurde für WB, ELISA und FM validiert. Er ist geeignet, MAD2L1 in Proben von Human zu detektieren.

Kurzübersicht für MAD2L1 Antikörper (AA 1-25) (ABIN129587)

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAD2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Produktdetails anti-MAD2L1 Antikörper

Bindungsspezifität: AA 1-25

Hersteller Produkt- Nr.: 600-401-461

Hersteller: Rockland

Verwendungszweck: MAD2L1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human MAD2L1 protein.

Produktmerkmale: Synonyms: rabbit anti-MAD2L1 antibody, MAD2, MAD2-like protein 1, HsMAD2, Mitotic arrest deficient 2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal region near amino acid residues 1-25 of Human MAD2L1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.19 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Andere Bezeichnung: MAD2L1

Hintergrund: Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.   This protein has a nuclear localization.

Gen-ID: 4085, 4505067

UniProt: Q13257