Biotin Antikörper (PE)

Produktnummer ABIN129563

Hersteller Produkt- Nr.: 600-108-098

Der Ziege Polyklonal Anti-Biotin-Antikörper wurde für WB, ELISA, IHC, FACS und FM validiert. Er ist geeignet, Biotin in Proben von zu detektieren.

Kurzübersicht für Biotin Antikörper (PE) (ABIN129563)

Target: Biotin

Reaktivität: Bitte anfragen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit PE

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Produktdetails anti-Biotin Antikörper (PE)

Hersteller Produkt- Nr.: 600-108-098

Hersteller: Rockland

Verwendungszweck: Biotin Antibody Phycoerythrin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Phycoerythrin, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Produktmerkmale: Synonyms: goat anti-Biotin Antibody PE Conjugation, Phycoerythrin conjugated goat anti-Biotin Antibody, Vitamin B4 antibody

Aufreinigung: Anti-Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: 1.0 μg/1,000,000 cells

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Biotin detects biotin and biocytin by ELISA, Immuno-histo-chemistry (IHC), and the antibody also recognizes biotin conjugated to various immunoglobulins. The antibody may be used in amplification techniques, immunoblotting, in situ nucleic acid hybridization, flow cytometry or Immunofluorescence microscopy.

Western Blot Dilution: 1:50-1:200

ELISA Dilution: 1:50-1:200

IF Microscopy Dilution: 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Haltbarkeit: 6 months

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Phycoerythrin Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.