MTBP Antikörper (AA 667-812)

Produktnummer ABIN129549

Hersteller Produkt- Nr.: 500-301-875

Dieses Anti-MTBP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MTBP in WB und ELISA. Geeignet für Human.

Kurzübersicht für MTBP Antikörper (AA 667-812) (ABIN129549)

Target: MTBP (MDM2 Binding Protein (MTBP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MTBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: PHL-1

Produktdetails anti-MTBP Antikörper

Bindungsspezifität: AA 667-812

Hersteller Produkt- Nr.: 500-301-875

Hersteller: Rockland

Verwendungszweck: MTBP Antibody

Kreuzreaktivität (Details): This antibody is directed against human MTBP protein.

Produktmerkmale: Synonyms: mouse anti-MTBP antibody, mouse anti-mouse double minute 2 antibody, MDM 2 BP antibody, MDM2 (mouse double minute 2) binding protein 104kD antibody, MDM2 (mouse double minute 2) binding protein antibody, MDM2 Binding Protein antibody, MDM2 BP antibody

Aufreinigung: The product was produced from clarified mouse ascetic fluid.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS_6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: This antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:2,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MTBP

Target: MTBP (MDM2 Binding Protein (MTBP))

Andere Bezeichnung: MTBP

Hintergrund: Background: The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor.  Studies also indicate that MDM2 regulates cell proliferation via p53 independent mechanisms.   MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1).  MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2).   The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).

Gen-ID: 27085

NCBI Accession: NP_071328

UniProt: Q96DY7