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ULP1 Antikörper (ULP1) Primary Antibody

ULP1 Reaktivität: Saccharomyces cerevisiae IP, IHC, ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN129522
$744.00
Zzgl. Versandkosten $45.00
500 μg
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 1 bis 2 Werktagen
  • Target Alle ULP1 Antikörper anzeigen
    ULP1
    Reaktivität
    Saccharomyces cerevisiae
    Wirt
    • 1
    Kaninchen
    Klonalität
    • 1
    Polyklonal
    Applikation
    Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
    Immunogentype:Recombinant
    Isotyp
    IgG
  • Applikationshinweise
    This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Restore with deionized water (or equivalent)
    Konzentration
    5.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    ULP1
    Andere Bezeichnung
    ULP1 (ULP1 Produkte)
    Synonyme
    NIB1, SUMO protease ULP1, SUMO deconjugating enzyme Ulp1, ULP1, ulp1
    Hintergrund
    ULP-1, ubiquitin-like protein-specific protease 1, initially processes Smt3 and also acts as a deconjugating enzyme for Smt3 [Saccharomyces cerevisiae (Baker's yeast)].  Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression.  But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization.  Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein.  Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.   Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3.  Three distinct steps can be distinguished in the SUMO modification pathway: 1) activation of SUMO, 2) transfer of SUMO to the conjugating enzyme, and 3) substrate modification. Since SUMO is synthesized as a precursor protein, a maturation step precedes the activation reaction. In yeast, C-terminal processing of the SUMO precursor is mediated by the processing protease Ulp1, which has an additional role in the deconjugation of SUMO-modified substrates. Mature SUMO is activated by SUMO-activating enzyme, an E1-like heterodimeric protein complex composed of Uba2 and Aos1.  Ulp1 function has provided evidence that SUMO modification in yeast, as has been suspected for vertebrates, plays an important role in nucleocytoplasmic trafficking.
    Synonyms: Probable sentrin specific protease antibody, Ubiquitin Like Protease antibody
    Gen-ID
    856087, 6325237
    UniProt
    Q02724
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