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BUBR1 Kinase Kinase (AA 1-350) Antikörper

Reaktivität: Human ELISA, FM, IP, WB Wirt: Maus Monoclonal 8G1 unconjugated
Rockland
Produktnummer ABIN129516
Hersteller Produkt- Nr.: 200-301-902
  • Target
    BUBR1 Kinase Kinase
    Bindungsspezifität
    AA 1-350
    Reaktivität
    Human
    Wirt
    Maus
    Klonalität
    Monoklonal
    Applikation
    ELISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Western Blotting (WB)
    Hersteller Produkt- Nr.
    200-301-902
    Hersteller
    Rockland
    Verwendungszweck
    BUBR1 Kinase Antibody
    Kreuzreaktivität (Details)
    This Protein A purified antibody is directed against human BUBR1 Kinase protein.
    Produktmerkmale
    Synonyms: mouse anti-BUBR1 antibody, Beta homolog of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1
    Aufreinigung
    The product was purified from tissue culture supernatant by chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.

    Immunogen Type: Recombinant Protein

    Klon
    8G1
    Isotyp
    IgG1
  • Applikationshinweise

    Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.  The use of HeLa whole cell lysates is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8.  Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1 % BSA.

    Western Blot Dilution: 1:200 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    IF Microscopy Dilution: 1:200 - 1:1,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Lázaro-Diéguez, Ispolatov, Müsch: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).

  • Target
    BUBR1 Kinase Kinase
    Hintergrund
    Background: BUBR1 Kinase (BUB1-related protein kinase) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores.  This protein is highly expressed in thymus followed by spleen.  Defects in BUB1B are associated with tumor formation.
    Gen-ID
    701
    NCBI Accession
    NP_001202
    UniProt
    O60566
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