BUBR1 Kinase Kinase (AA 1-350) Antikörper

Produktnummer ABIN129516

Hersteller Produkt- Nr.: 200-301-902

Produktdetails

Target: BUBR1 Kinase Kinase

Bindungsspezifität: AA 1-350

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: ELISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Western Blotting (WB)

Hersteller Produkt- Nr.: 200-301-902

Hersteller: Rockland

Verwendungszweck: BUBR1 Kinase Antibody

Kreuzreaktivität (Details): This Protein A purified antibody is directed against human BUBR1 Kinase protein.

Produktmerkmale: Synonyms: mouse anti-BUBR1 antibody, Beta homolog of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1

Aufreinigung: The product was purified from tissue culture supernatant by chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.

Immunogen Type: Recombinant Protein

Klon: 8G1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.  The use of HeLa whole cell lysates is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8.  Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1 % BSA.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:200 - 1:1,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Lázaro-Diéguez, Ispolatov, Müsch: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).

Antigendetails

Target: BUBR1 Kinase Kinase

Hintergrund: Background: BUBR1 Kinase (BUB1-related protein kinase) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores.  This protein is highly expressed in thymus followed by spleen.  Defects in BUB1B are associated with tumor formation.

Gen-ID: 701

NCBI Accession: NP_001202

UniProt: O60566