MAPK8 / MAPK9 (pThr183), (pTyr185) Antikörper

Produktnummer ABIN127216

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MAPK8 / MAPK9 in WB, IHC (p), IF und IP. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für MAPK8 / MAPK9 (pThr183), (pTyr185) Antikörper (ABIN127216)

Target: MAPK8 / MAPK9

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails

Bindungsspezifität: pThr183, pTyr185

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of Human SAPK/JNK

Anwendungsinformationen

Applikationshinweise: Western Blot: 1-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunocytochemistry: 10-20 μg/mL. Immunohistochemistry: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % Glycerol, 1 % BSA and 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Antigendetails

Target: MAPK8 / MAPK9

Hintergrund: The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.Synonyms: JNK-1, JNK-2, JNK-46, JNK-55, JNK1, JNK2, MAPK-8, MAPK-9, MAPK8, MAPK9, Mitogen-activated protein kinase 8/9, PRKM8, PRKM9, SAPK, Stress-activated protein kinase JNK11/JNK2, c-Jun N-terminal kinase 1/2