OCT4 Antikörper (N-Term)

Produktnummer ABIN127203

Produktdetails anti-OCT4 Antikörper

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide mapping to the N-terminus of mouse Oct-4.

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-4 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL. Positive Control: Rat kidney tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 30 % Glycerol, 0.5 % BSA

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for longer.

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: OCT3/4 (POU5F1 Produkte)

Hintergrund: Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1

Gen-ID: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance