1: 50 dilutions for nonradioactive EIA assays (10 μL/assay in an 70 μL assay reaction). Thematerial is sufficient for performing 500 nonradioactive EIA assays or 6,000 radioactiveassays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Nur für Forschungszwecke einsetzbar
PBS, 0.02 % Sodium Azide, 50 % Glycerol, 1 % BSA
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Avoid repeated freezing and thawing.
-20 °C/-80 °C
Informationen zur Lagerung
Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.