NSF Antikörper

Produktnummer ABIN127184

Der Kaninchen Polyklonal Anti-NSF-Antikörper wurde für WB, IP und IHC (p) validiert. Er ist geeignet, NSF in Proben von Ratte und Maus zu detektieren.

Kurzübersicht für NSF Antikörper (ABIN127184)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NSF Antikörper

Spezifität: The antibody recognizes 85 kDa NSF.

Aufreinigung: Protein A chromatography

Immunogen: Synthetic peptide corresponding to amino acids around 731 of rat NSF

Anwendungsinformationen

Applikationshinweise: Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-15 mg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.

Details zu NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Andere Bezeichnung: NSF

Hintergrund: Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.Synonyms: N-ethylmaleimide-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

Gen-ID: 60355

NCBI Accession: NP_068516

UniProt: Q9QUL6