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YES1 Antikörper (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1) N-Term Primary Antibody

YES1 Reaktivität: Human EIA, WB Wirt: Maus Monoclonal 2B8 unconjugated
Produktnummer ABIN126915
$663.30
Zzgl. Versandkosten $45.00
0.1 mg
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Available
  • Target Alle YES1 Antikörper anzeigen
    YES1
    Bindungsspezifität
    • 21
    • 18
    • 16
    • 15
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 102
    • 41
    • 14
    • 3
    • 1
    • 1
    Human
    Wirt
    • 93
    • 11
    Maus
    Klonalität
    • 95
    • 8
    Monoklonal
    Konjugat
    • 39
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser YES1 Antikörper ist unkonjugiert
    Applikation
    • 67
    • 48
    • 26
    • 26
    • 25
    • 14
    • 9
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Enzyme Immunoassay (EIA), Western Blotting (WB)
    Spezifität
    AM00159PU-N specifically recognizes Yes at 59 kDa.
    Produktmerkmale
    Synonyms: YES, p61-Yes, c-Yes, Proto-oncogene tyrosine-protein kinase Yes
    Aufreinigung
    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
    Bestandteile
    incl. positive Control
    Immunogen
    Synthetic peptide conjugated to KLH
    Klon
    2B8
    Isotyp
    IgG1
  • Applikationshinweise
    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Use A431 untreated cell lysate as Positive Control. ELISA: 0.05 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    Lyophilized Cell Lysate from serum starved A431 cellsReconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control is recommended for immunoblot applications. 20 µl of positive controlcell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation!
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
    Haltbarkeit
    12 months
  • Target
    YES1
    Andere Bezeichnung
    YES1 (YES1 Produkte)
    Synonyme
    HsT441, P61-YES, Yes, c-yes, yes, p61-Yes, hst441, p61-yes, YES, c-Yes, p60c-yes, YES proto-oncogene 1, Src family tyrosine kinase, YES proto-oncogene 1, Src family tyrosine kinase L homeolog, YES1, yes1.L, yes1, Yes1
    Hintergrund
    Yes is a member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Yes, src and fyn are ubiquitously expressed while the other members are primarily expressed in hematopoietic cells. Each protein tyrosine kinase (PTK) contains three domain (SH1, SH2, SH3). The SH1 domain has PTK activity, while the SH2 and SH3 domains are invloved in mediating protein-protein interactions by binding phosphotyrosine- containing and proline-riche motifs. By means of SH2-phosphotyrosine binding, PTKs are able to associate with activated receptor protein tyrosine kinases, e.g. platelet-derived growth factor (PDGFR)- and epidermal growth factor (EGF)- receptor.Synonyms: Proto-oncogene tyrosine-protein kinase Yes, YES, c-Yes, p61-Yes
    Gen-ID
    7525
    UniProt
    P07947
    Pathways
    CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
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