Collagen Type I Antikörper

Produktnummer ABIN126040

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Collagen Type I in IHC (fro), IF und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Collagen Type I Antikörper (ABIN126040)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-Collagen Type I Antikörper

Spezifität: This antibody detects exhibits the following reactivities in ELISA both native and denaturated Collagen types: Collagen types 1 and 3: 100 % . Collagen types 4 and 5: 90 %. Collagen type 2: 80 %. Fibronectin, Albumin and Immunoglobulins all < 0.5 %.

Kreuzreaktivität (Details): Also reacts to: type II, and V, III, IV

Aufreinigung: Affinity Chromatography on immobilized collagen types I-V

Immunogen: A mixture of human collagen 1-5 from human placenta.

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections (1/10-1/40). ELISA (1/2000-1/8000). Immunoflourescence (1/10-1/40).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.5 containing 0.1 % Mannitol and 0.1 % Dextran and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen Type I

Hintergrund: Collagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte. Collagen III occurs in most soft connective tissues along with collagen I. Collagen IV is the major structural component of glomerular basement membranes, forming a Chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gen-ID: 1277

NCBI Accession: NP_000079

UniProt: P02452