KRT20 Antikörper

Produktnummer ABIN125990

Der Maus Monoklonal anti-KRT20 Antikörper wird verwendet zum Nachweis von KRT20 in Proben von Human und Schwein. Er wurde validiert für WB und IHC (fro).

Kurzübersicht für KRT20 Antikörper (ABIN125990)

Target: KRT20 (Keratin 20 (KRT20))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: KS20-5

Produktdetails anti-KRT20 Antikörper

Spezifität: IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reacts with human, pig, not with rat. Reacts with HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

Aufreinigung: Protein A affinity chromatography

Immunogen: Electrophoretically purified keratin K20 from human intestinal mucosa.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemical on frozen sections: 1: 10, 1h at RT or over night at 2-8C. Immunohistochemical on cytological material. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL dist. water

Buffer: PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu KRT20

Target: KRT20 (Keratin 20 (KRT20))

Andere Bezeichnung: Cytokeratin 20

Hintergrund: Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT

Gen-ID: 54474

NCBI Accession: NP_061883

UniProt: P35900