Thylakoid Lumen Cytchrome C6 Protein (Cyt C6) Antikörper

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Antigen
Reaktivität
Chlamydomonas reinhardtii (C. reinhardtii)
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
Optionen
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Immunogen GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6
Kreuzreaktivität (Details) Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Homologie Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata
Produktmerkmale Expected / apparent Molecular Weight of the Antigene: 15 kDa
Reinigung serum
Hintergrund Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.. Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.
Molekulargewicht 15 kDa
UniProt A8J9G6
Applikationshinweise 1 : 1 000 with alkaline phosphatase (WB)
Kommentare

Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 200 μL of sterile water.
Handhabung Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Allgemeine Veröffentlichungen Kropat, Gallaher, Urzica, Nakamoto, Strenkert, Tottey, Mason, Merchant: "Copper economy in Chlamydomonas: prioritized allocation and reallocation of copper to respiration vs. photosynthesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 9, pp. 2644-51, 2015 (PubMed).

Quinn, Merchant: "Copper-responsive gene expression during adaptation to copper deficiency." in: Methods in enzymology, Vol. 297, pp. 263-79, 1998 (PubMed).

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