Psbd Antikörper (C-Term)

Produktnummer ABIN125883

Zitiert in 5+ Publikationen. Der Kaninchen Polyklonal anti-Psbd Antikörper (ABIN125883) detektiert spezifisch Psbd in CN-PAGE und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Nicotiana tabacum, Mais (Zea mays), Ulva prolifera, Triticosecale, Trifolium pratense, Thalassiosira pseudonana, Thalassiosira guillardii, Synechocystis, Synechococcus sp., Sinapis alba, Phaeodactylum tricornutum, Pea, Oryza sativa, Anabaena variabilis, Lotus corniculatus, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Green bean, Emiliania huxleyi, Echinochloa crus-galli, Ditylum brightwellii, Diatom (Skeletonema), Cyanobakterien, Chromochloris zofingiensis, Chlamydomonas reinhardtii und Arabidopsis thaliana.

Kurzübersicht für Psbd Antikörper (C-Term) (ABIN125883)

Target: Psbd (D2 Protein of Psii (Psbd))

Reaktivität: Nicotiana tabacum, Mais (Zea mays), Ulva prolifera, Triticosecale, Trifolium pratense, Thalassiosira pseudonana, Thalassiosira guillardii, Synechocystis, Synechococcus sp., Sinapis alba, Phaeodactylum tricornutum, Pea, Oryza sativa, Anabaena variabilis, Lotus corniculatus, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Green bean, Emiliania huxleyi, Echinochloa crus-galli, Ditylum brightwellii, Diatom (Skeletonema), Cyanobakterien, Chromochloris zofingiensis, Chlamydomonas reinhardtii, Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Clear Native PAGE (CN-PAGE), Western Blotting (WB)

Produktdetails anti-Psbd Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-PsbD | D2 protein of PSII

Spezifität: Perfect across all full-length PsbD sequences from higherplants, lowerplants, cyanobacteria and unicellular algae except: -minorsubstitutions in some Prochlorococcus & Dinoflagellatesequences. The antibody should still work against these taxa,but it has not been tested yet. This antibody does not detectPsbA protein (D1).

Kreuzreaktivität (Details): Arabidopsis thaliana, Anabaena 7120, Ditylum brightwellii, Horderum vulgare, Chlamydomonas reinhardtii, Chromochloris zofingiensis, Echinola crus-galli, Emiliania huxleyi, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, picocyanobacteria, Pisum sativum, Phaseolus vulgaris, Phaeodactylum tricornutum, Trifolium pratense, Skeletonema costatum (diatom), Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Thalassiosira guillardii, Thalassiosira pseudonana, Triticale, Ulva prolifera, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Aegilops tauschii,Brassica napus,Cannabis sativa,Capsicum annuum,Centrolepsis monogyna,Chromera velia,Crocosphaera watsonii,Cyanidioschyzon merolae,Fischerella sp.,Galdieria sulphuraria,Glycine max,Glycine soja,Leiosporoceros dussii,Cucumis sativa,Manihot esculenta,Marchantia polymorpha,Microcystis aeruginosa,Nannochloropsis,Panax ginseng,Petermannia cirrosa,Pinus thunbergii,Physcomitrium patens,Pinus strobus,Populus trichocarpa,Ricinus communis,Solanum tuberosum,Spinacia oleracea,Solanum lycopersicum. Triticum aestivum,Utricularia alpina,Vitis vinifera,Vitrella brassicaformis

Aufreinigung: Serum

Immunogen: KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis thaliana P56761

Anwendungsinformationen

Applikationshinweise: 1: 10 000 (CN-PAGE), 1: 5000 - 1 : 50 000 (WB)

Kommentare:

There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii.For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-Psbd Antikörper (ABIN125883)

Mou, Zhang, Dong, Fan, Xu, Cao, Xu, Wang, Ye: "Photoprotection in the green tidal alga Ulva prolifera: role of LHCSR and PsbS proteins in response to high light stress." in: Plant biology (Stuttgart, Germany), (2013) (PubMed).

Signorelli, Casaretto, Sainz, Díaz, Monza, Borsani: "Antioxidant and photosystem II responses contribute to explain the drought-heat contrasting tolerance of two forage legumes." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 70, pp. 195-203, (2013) (PubMed).

Yamatani, Sato, Masuda, Kato, Morita, Fukunaga, Nagamura, Nishimura, Sakamoto, Tanaka, Kusaba: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).

Latijnhouwers, Xu, Muoller: "Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development." in: Planta, (2010) (PubMed).

Perreault, Dionne, Didur, Juneau, Popovic: "Effect of cadmium on photosystem II activity in Chlamydomonas reinhardtii: alteration of O-J-I-P fluorescence transients indicating the change of apparent activation energies within photosystem II." in: Photosynthesis research, (2010) (PubMed).

Details zu Psbd

Target: Psbd (D2 Protein of Psii (Psbd))

Hintergrund:

Background: D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39.5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.

Additional information: The peptide used to elicit this antibody has a perfect conservation across all full-length PsbD sequences from higher plants, lower plants, cyanobacteria and unicellular algae except: minor substitutions in some Prochlorococcus & Dinoflagellate sequences, The antibody should still work against these taxa, but it has not been tested yet, This antibody does not detect PsbA protein (D1),This product can be sold containing ProClin if requested

Molekulargewicht: 39.4 | 28-30 kDa

Gen-ID: 844775

UniProt: P56761, P11849, P06007, P20898