Vitellogenin 2 Antikörper

Produktnummer ABIN125873

Der Kaninchen Polyklonal anti-Vitellogenin 2 Antikörper wird verwendet zum Nachweis von Vitellogenin 2 in Proben von Sea bass (Dicentrarchus labrax), Senegalese sole und Sparus aurata. Er wurde validiert für ELISA und WB.

Kurzübersicht für Vitellogenin 2 Antikörper (ABIN125873)

Target: Vitellogenin 2

Reaktivität: Sea bass (Dicentrarchus labrax), Senegalese sole, Sparus aurata 

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-VTG | Sole vitellogenin

Kreuzreaktivität (Details): Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Aufreinigung: Serum

Immunogen: Native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)

Anwendungsinformationen

Applikationshinweise: 1 : 5 000 on sole serum (ELISA), 1 : 5 000 (WB)

Kommentare:

the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: Vitellogenin 2

Andere Bezeichnung: VTG

Hintergrund:

Background: Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.

Additional information: VTG can be purified using following methodology: Mañanós et al. (1994). Sea bass (Dicentrarchus labrax L.) vitellogenin. I-Induction, purification and partial characterization. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol 107 (2): 205-216.Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.

Molekulargewicht: ca. 200 kDa