Vitellogenin 2 Antikörper

Details zu Produkt Nr. ABIN125873, Anbieter: Anmelden zum Anzeigen
Antigen
  • VIT2
  • vit
  • vitellogenin-2
  • vitellogenin 2
  • VTG2
Reaktivität
Sea bass (Dicentrarchus labrax)
12
1
Wirt
Kaninchen
12
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
3
3
3
Applikation
ELISA, Western Blotting (WB)
9
8
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)
Homologie n. a.
Reinigung serum
Andere Bezeichnung VTG
Hintergrund Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.
Applikations-hinweise Recommended Dilution: 1 : 5 000 (WB), 1 : 5 000 on sole serum (ELISA).
Kommentare

the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 200 µL of sterile water
Handhabung Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-Vitellogenin 2 antibody (ABIN125873) Samples.STD: Protein standard (molecular weights of the proteins are indicated on the...
Allgemeine Veröffentlichungen Guzmán, Norberg, Ramos, Mylonas, Mañanós: "Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis)." in: General and comparative endocrinology, Vol. 156, Issue 2, pp. 285-97, 2008 (PubMed).

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