No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus PCC 7942
Expected / apparent Molecular Weight of the Antigene: 24 kDa / 17 kDa for Arabidopsis thaliana
KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.
Recommended Dilution: 1 : 1000 with standard ECL (WB).
Nur für Forschungszwecke einsetzbar
For reconstitution add 200 µL of sterile water
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Sakuraba, Rahman, Cho, Kim, Koh, Yoo, Paek: "The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 1, pp. 122-33, (2013) (PubMed).
Krech, Ruf, Masduki, Thiele, Bednarczyk, Albus, Tiller, Hasse, Schöttler, Bock: "The plastid genome-encoded Ycf4 protein functions as a nonessential assembly factor for photosystem I in higher plants." in: Plant physiology, Vol. 159, Issue 2, pp. 579-91, (2012) (PubMed).
AGI Code: At1g31330 PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.
expected: 24 kDa , apparent: 17 kDa for Arabidopsis thaliana