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PRDX6-PS2 Antikörper (Peroxiredoxin 6 Pseudogene 2) C-Term Primary Antibody

PRDX6-PS2 Reaktivität: Arabidopsis thaliana ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Pubmed (2 references)
Produktnummer ABIN125822
$617.35
Zzgl. Versandkosten $45.00
100 μL
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 6 bis 8 Werktagen
  • Target
    PRDX6-PS2
    Bindungsspezifität
    C-Term
    Reaktivität
    Arabidopsis thaliana
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    ELISA, Western Blotting (WB)
    Kreuzreaktivität (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
    Aufreinigung
    serum
    Immunogen
    thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
  • Applikationshinweise
    1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
    Kommentare

    This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 100 μL of sterile water.
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).

    Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).

  • Target
    PRDX6-PS2
    Andere Bezeichnung
    GPX
    Hintergrund
    AGI Code: At4g31870
    GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
    Molekulargewicht
    expected: 25 kDa, apparent: 20 kDa
    UniProt
    Q9SZ54
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