Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2) (C-Term) Antikörper

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Antigen
Epitop
C-Term
Reaktivität
Arabidopsis thaliana
1
1
Wirt
Kaninchen
1
Klonalität
Polyklonal
Applikation
ELISA, Western Blotting (WB)
1
1
1
Optionen
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Immunogen thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
Kreuzreaktivität (Details) Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Produktmerkmale Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
Reinigung serum
Andere Bezeichnung GPX
Hintergrund AGI Code: At4g31870
GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
Molekulargewicht expected: 25 kDa, apparent: 20 kDa
UniProt Q9SZ54
Applikations-hinweise 1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
Kommentare

This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 100 μL of sterile water.
Handhabung Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Produkt verwendet in: Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, 2013 (PubMed).

Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, 2010 (PubMed).

Allgemeine Veröffentlichungen Juszczak, Rudnik, Pietzenuk, Baier: "Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions." in: Physiologia plantarum, Vol. 146, Issue 1, pp. 53-70, 2012 (PubMed).

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