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PRDX6-PS2 Antikörper (C-Term)

Der Kaninchen Polyklonal Anti-PRDX6-PS2-Antikörper wurde für ELISA und WB validiert. Er ist geeignet, PRDX6-PS2 in Proben von Arabidopsis thaliana zu detektieren. Es sind 2+ Publikationen verfügbar.
Produktnummer ABIN125822

Kurzübersicht für PRDX6-PS2 Antikörper (C-Term) (ABIN125822)

Target

PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))

Reaktivität

Arabidopsis thaliana

Wirt

Kaninchen

Klonalität

Polyklonal

Applikation

ELISA, Western Blotting (WB)
  • Bindungsspezifität

    C-Term

    Kreuzreaktivität (Details)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa

    Aufreinigung

    serum

    Immunogen

    thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
  • Applikationshinweise

    1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)

    Kommentare

    This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 100 μL of sterile water.

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).

    Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).

  • Target

    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))

    Andere Bezeichnung

    GPX

    Hintergrund

    AGI Code: At4g31870
    GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.

    Molekulargewicht

    expected: 25 kDa, apparent: 20 kDa

    UniProt

    Q9SZ54
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