PRDX6-PS2 Antikörper (C-Term)

Produktnummer ABIN125822

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-PRDX6-PS2 Antikörper wird verwendet zum Nachweis von PRDX6-PS2 in Proben von Arabidopsis thaliana. Er wurde validiert für ELISA und WB.

Kurzübersicht für PRDX6-PS2 Antikörper (C-Term) (ABIN125822)

Target: PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-PRDX6-PS2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-GPX | Glutathione peroxidase (chloroplastic)

Kreuzreaktivität (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Aufreinigung: Serum

Immunogen: thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production

Anwendungsinformationen

Applikationshinweise: 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)

Kommentare:

This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-PRDX6-PS2 Antikörper (ABIN125822)

Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).

Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).

Details zu PRDX6-PS2

Target: PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))

Andere Bezeichnung: GPX

Hintergrund: GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.

Molekulargewicht: 25 | 20 kDa

Gen-ID: 829316

UniProt: Q9SZ54